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(ÀúÀÚ¿Í ¼Ò¼Ó Ç¥±âÀÇ ¿¹),

  • Kam Chan Kangp1, Gil-Dong Hong1, and Chun-Hyang Sungc12

    1Department of Molecular Biology, Hankook University, Seoul 02850; 2LMO Evalution Laboratory, Korea Research Institute of Bioscience and Biotechnology, Deajeon 34141

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ÃÊ·Ï ¿¹

Proteomic Analysis of Encystment Related Proteins in Acanthamoeba castellanii

Kam Chan Kangp, Chun-Hyang Sungc

Department of Molecular Biology, Hankook University, Seoul 02850

Acanthamoeba castellanii have two distinct forms, trophozoite and cyst. When deprived of nutrients or under other stresses, trophozoites undergo a cellular differentiation into cysts. To find the factors mediating encystation and analyze its mechanism, we carried out a proteomic analysis. In total, 48 proteins were identified by MALDI-TOF MS and 11 proteins of these proteins were determined as stage-specific markers. Numerous proteins were differently regulated during encystment, including decrease of cytoskeleton expression, degradation of S-adenosylmethionine synthetase and increase of several defense-related proteins. By 2-D blotting assay, we immunologically identified actins, ubiquitin and HSP70 and found appearance of fragmented actins in mature cyst. Of three immunologically identified proteins, actins were confirmed as A. castellanii actin-1 by MALDI-TOF MS. We concluded that these proteins play an important role in cyst maturation by regulating the ubiquitin-dependent proteolysis system. This study provides some critical clues for understanding cellular differentiation in Acanthamoeba castellanii as a model organism.

* This work was supported by KOSEF grant
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